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Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional <t>reconstructed</t> <t>micro-CT</t> images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).
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Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional <t>reconstructed</t> <t>micro-CT</t> images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).
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Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional <t>reconstructed</t> <t>micro-CT</t> images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).
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Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional <t>reconstructed</t> <t>micro-CT</t> images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).
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Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional <t>reconstructed</t> <t>micro-CT</t> images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).
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clk-1276  (Jena Bioscience)
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Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional <t>reconstructed</t> <t>micro-CT</t> images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).
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Image Search Results


Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional reconstructed micro-CT images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).

Journal: International Journal of Nanomedicine

Article Title: Sequential Release of mRNA Complex and T Cells by a Double-Layered Implantable Scaffold for Combination Therapy of Head and Neck Squamous Cell Carcinoma

doi: 10.2147/IJN.S582078

Figure Lengend Snippet: Treatment effect of double-layered scaffold in C3H/HeN mandible-invasion models. ( A ) Illustration of the experimental process schedule and implantation operation of the scaffold in the mandible-invasion model. ( B ) Weight of mice during the experiments (ns, not significant). ( C ) Tumor growth curve measured in each group with different treatments (****P<0.0001, **P<0.01). ( D ) Tumor growth tendency in different groups. ( E ) Representative images of the masseter region of mice from each group. ( F ) Weight of tumors in each group at the end of the experiment (**P<0.01, *P<0.05). ( G ) Three-dimensional reconstructed micro-CT images of the skulls of mice in each group, including side and top views. ( H ) The calculated bone volume fraction (BV/TV) at the end of the experiment in each group and zygomatic bone destruction scores in each group (***P<0.001, **P<0.01, *P<0.05). ( I ) Representative flow cytometry images and quantification of T cells and DCs in the tumor after treatment with the double-layered scaffold (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).

Article Snippet: In addition, micro-computed tomography (micro-CT) imaging (SkyScan 1276, Bruker-microCT, Kontich, Belgium) was performed during the study and post-sample collection (70 kV, 200 μA, pixel size 15 μm, 2048×2048 resolution, 1 mm filter, 0.4 degrees rotation step) under isoflurane inhalation anesthesia.

Techniques: Micro-CT, Flow Cytometry